Development of Broad Spectrum and Durable Bacterial Blight Resistant Variety through Pyramiding of Four Resistance Genes in Rice

Bacterial blight (BB) disease caused by Xanthomonas oryzae pv. is a major biotic constraint on obtaining higher grain yields in rice. Marker-assisted backcross breeding (MABB) was performed the pyramiding of Xa4, xa5, xa13 and Xa21 resistance genes popular variety, Ranidhan. A foreground selection BC1F1, BC2F1, BC3F1 progenies detected all target 12, 7 16 using closely linked markers from population size 446, 410, 530, respectively. The BB-positive carrying with maximal similarity to recipient parent backcrossed each generation. total 1784 BC3F2 seeds were obtained best progeny. screening for four resulted eight plants genes. bioassay pyramided lines conferred very high levels predominant isolates bacterial disease. In addition, these similar Ranidhan morpho-quality traits, namely, plant height, filled grains/panicle, panicles/plant, length, breadth, weight, milling, head rice recovery, kernel length after cooking, water uptake, volume expansion ratio, gel consistency, alkali-spreading value, amylose content.